13C代謝流和定量代謝物組學(xué)研究團(tuán)隊(duì)
利用本實(shí)驗(yàn)室先進(jìn)的微型反應(yīng)器、快速取樣裝置和高效液相/氣相質(zhì)譜儀可以同時(shí)實(shí)現(xiàn)胞內(nèi)代謝物濃度的測(cè)定和13C代謝流的計(jì)算，從而為以13C代謝流為核心的縱向組學(xué)整合技術(shù)打下堅(jiān)實(shí)基礎(chǔ)。并與宏觀過(guò)程生物生理代謝參數(shù)OUR、CER、RQ等相結(jié)合分析，實(shí)驗(yàn)發(fā)酵過(guò)程的全局優(yōu)化。
快速取樣、淬滅和提取裝置示意圖
13C代謝流和定量代謝物組測(cè)定流程圖
13C代謝流和定量代謝物組學(xué)在黑曲霉生產(chǎn)糖化酶過(guò)程中的應(yīng)用
研究成果
1.Impacts of high β-galactosidase expression on central metabolism of recombinant Pichia pastoris GS115 using glucose as sole carbon source via13C metabolic flux analysis. Journal of Biotechnology, 2014, 187: 124-134.
2.Integrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger. Microbial Cell Factories, 2015, 14: 147-160.
3.Enhanced L-lactic acid production in Lactobacillus paracasei by exogenous proline addition based on comparative metabolite profiling analysis. Applied Microbiology and Biotechnology, 2016, 100: 2301-2310.